In the last several years the concept of using a gonadotropin releasing hormone agonist GnRH-a for triggering ovulation in patients treated by an antagonist protocol for IVF became almost a routine clinical practice. It may promote oocyte nuclear maturation, resumption of meiosis and cumulus expansion. Several studies reported the retrieval of more mature oocytes after GnRH-a, therefore it seems that this attempt could be beneficial in an in vitro maturation IVM oocyte cycle performed for fertility preservation in patients with malignancies but possible for other indication as polycystic ovarian syndrome patients. We presented a case of a patient needed fertility preservation that underwent 3 IVM cycles priming ovulation with a GnRH-a. Twelve oocytes were obtained, all of them matured 4.
The goal was to evaluate the role of the number of retrieved immature oocytes on mature oocyte counts and morphology, and also the rates of fertilization and embryo development in ICSI cycles. In sub-analysis, the impacts of the number of GV and MI oocytes were assessed on the rates of fertilization and embryo development. Also, correlations between the numbers of immature and mature oocytes, as well as maternal age between two groups were analyzed. Assessments of oocyte morphology, fertilization, embryo quality and development were done accordingly.
Strassburger, S. Friedler, A. Raziel, E. Kasterstein, M. Schachter, R.
The aim of this study was to investigate which factors contribute to the incidence of immature oocytes germinal vesicle -GV- and metaphase I -MI- and how they impact the intracytoplasmic sperm injection ICSI outcomes of sibling mature oocytes. Cycles above a Our evidence suggests that oocytes derived from a cohort with high incidence of maturation fail may have detrimental clinical outcomes. By the time of birth, the human ovary contains a pool of quiescent primordial follicles, each consisting of a small inactive oocyte, arrested in the germinal vesicle GV stage, and a single layer of granulosa cells; the fate of each follicle is controlled by endocrine as well as paracrine factors Findlay et al. Of the millions of the primordial oocytes present at birth, only approximately mature during a woman's lifetime Monniaux et al.